Feed: Science Yahoo UK
Posted on: Monday, April 15, 2013 01:27
Author: Science Yahoo UK
Subject: Kidney grafts 'functioning in rats'
Kidneys have been fashioned by scientists and observed functioning in rats. A first step has also been made towards constructing human kidneys in the laboratory. Scientists used a similar approach already employed to produce bioengineered parts of hearts, lungs and livers. The US team first stripped living cells from donor organs with a detergent solution, leaving a "scaffold" of collagen connective tissue. The scaffold was then seeded with kidney cells from newborn rats, and human endothelial cells to replace the lining of blood vessels. Next the organs were cultured for up to 12 days in a "bioreactor" while being nourished with oxygen and nutrients. During this time, the seedling cells grew and spread. Laboratory tests showed that the constructed kidneys were able to filter blood and produce urine. Transplanted into living rats, they continued to produce urine with no evidence of bleeding or clot formation. Study leader Dr Harald Ott, from Massachusetts General Hospital, whose work is reported in the journal Nature Medicine, said: "What is unique about this approach is that the native organ's architecture is preserved, so that the resulting graft can be transplanted just like a donor kidney and connected to the recipient's vascular and urinary systems. If this technology can be scaled to human-sized grafts, patients suffering from renal failure who are currently waiting for donor kidneys or who are not transplant candidates could theoretically receive new organs derived from their own cells." The scientists have made a start to applying the technology at larger scales by stripping cells from pig and human kidneys. In rats, the performance of the regenerated organs was significantly lower than that of normal, healthy kidneys. This could be due to the immaturity of the newborn cells used to repopulate the organ scaffolding, the researchers believe. Demand for transplant kidneys in the UK is far higher than the available supply of donated organs, both living and dead. Between April 2010 and April 2011 a total of 1,020 living kidney donations were made, and 1,667 organs were taken from recently deceased people. But this still left just under 7,000 people on the waiting list for a donation. People can survive happily with just one kidney, but kidney failure affecting both organs requires either a transplant or dialysis, during which blood is filtered outside the body. |
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